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Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.12710/24272
Title: Experimental study in obtaining of a vascularised composite bone extracellular matrix
Authors: Stoian, Alina
Pavlovschi, Elena
Verega, Grigore
Birgit, Andree
Hilfiker, Andres
Nacu, Viorel
Keywords: extracellular bone matrix;decellularization;composite graft
Issue Date: 2023
Publisher: CEP Medicina
Citation: STOIAN, Alina, PAVLOVSCHI, Elena, VEREGA, Grigore, et al. Experimental study in obtaining of a vascularised composite bone extracellular matrix. In: Cells and Tissues Transplantation. Actualities and Perspectives: the materials of the national scientific conf. with internat. participation, Chișinău: CEP Medicina. 2023, p.40. ISBN 978-9975-82-313-5.
Abstract: Introduction. One of the final products of tissue engineering is the extracellular matrix (ECM), a noncellular component of tissues that can be obtained using different methods of decellularization. Most decellularization protocols are divided into those for soft tissues and those for hard tissues [1-4]. Our study aims to develop and test the universal protocol for decellularization of the composite vascularized bone graft (soft and hard tissue) in order to obtain the vascularized extracellular bone matrix that can be used in reconstruction of the massive bone defects. Material and Methods. The same protocol was used for the decellularization of different diameters vascular grafts, and different structures of bone tissue (soft and hard tissue) porcine origin. Like large diameter vessel, was was taken from the tibial bone. The efficacy of the protocol was demonstrated by histological examinations, DNA quantification and the biocompatibility test. Results. The used protocol has been effective even for small diameter vascular grafts and on cortical and spongy bone blocks. Histological examination (H&E staining) showed cell death after processing. DNA quantification has shown a decrease in the amount of DNA, especially for spongy bone grafts and the biocompatibility test has demonstrated the biocompatibility of vascular and bone grafts after processing. Conclusions: We can obtain an effective decellularization for both soft and hard tissues using the same protocol.
metadata.dc.relation.ispartof: „Cells and tissues transplantation. Actualities and perspectives” dedicated to the 10th anniversary of the founding of the Human Tissue and Cells Bank and to the 15th anniversary of the founding of the Laboratory of Tissue Engineering and Cells Culture of Nicolae Testemitanu State University of Medicine and Pharmacy of the Republic of Moldova, March 17-18th 2023, Chisinau, Republic of Moldova
URI: http://repository.usmf.md/handle/20.500.12710/24272
ISBN: 978-9975-82-313-5
Appears in Collections:The Materials of the National Scientific Conference with International Participation „Cells and tissues transplantation. Actualities and perspectives”

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