Abstract:
Introduction. The pharmaceutical nanotechnologies represent one of the most progressive and
promising branches of pharmaceutical technology. They permit the modification and directing of
the properties of active substances without the modification of chemical structure. For the new
nano-drug formulations, exists the need of implementation of new methods of analysis that will
take into account not only the chemical structure of the substance, but also the macro-molecular
formulation and its parameters.
Aim of the study. The aim of the study was the literature review of the existing methods for the
analysis of liposome formulation and their application to a specific formulation with antibiotic
substance.
Materials and methods. A literature review of existing methods of analysis of liposomes was
performed. The methods of analysis were classified in classes, depending of the studied
formulation parameter. If possible, the method was applied for the study of the parameters of the
formulated liposomes with antibacterial substances.
Results. In order to establish the existing methods of analysis of for liposomes, a study review of
94 literature references was performed (basically from PubMed and Medline library). The
methods of analysis were classified in 3 classes: optical, chemical and physico-chemical
methods. Using some of these methods, that were applicable to our study object, the parameters
of a prepared liposomic formulation were established. The methods were tested for linearity,
repeatability and reproducibility.
Conclusions. The results have shown that a part of classical methods for analysis of a liposomic
formulation sometimes are not enough to describe the studied preparation and to predict its
efficiency. Also, different liposomic formulations have demonstrated different results at the
application of the same method of analysis. This fact demonstrates the need of elaboration of
specific technics of analysis for each individual liposomic formulation.
