Institutional Repository in Medical Sciences
(IRMS – Nicolae Testemițanu SUMPh)

Obtaining of a suitable osteochondral graft for articular cartilage engineering

Show simple item record

dc.contributor.author Jian, Mariana
dc.contributor.author Cobzac, Vitalie
dc.date.accessioned 2020-07-06T07:44:08Z
dc.date.available 2020-07-06T07:44:08Z
dc.date.issued 2018
dc.identifier.citation JIAN, Mariana, COBZAC, Vitalie. Obtaining of a suitable osteochondral graft for articular cartilage engineering. In: MedEspera: the 7th Internat. Medical Congress for Students and Young Doctors: abstract book. Chișinău: S. n., 2018, p. 202-203. en_US
dc.identifier.uri https://medespera.asr.md/wp-content/uploads/Abastract-Book-2018.pdf
dc.identifier.uri http://repository.usmf.md/handle/20.500.12710/10979
dc.description Laboratory of Tissue Engineering and Cells Cultures, Laboratory of genetics, Nicolae Testemitanu State University of Medicine and Pharmacy of the Republic of Moldova Department of Biomedical Sciences. Faculty of Medical Bioengineering “Grigore T.Popa" University of Medicine and Pharmacy, Iasi, Romania en_US
dc.description.abstract Introduction. Chondral injuries are common following a knee trauma. There are numerous studies with different ways to obtain a suitable graft for articular cartilage regeneration, but without imposing results. Material and methods. From two freshly sacrificed rabbits the distal femurs were harvested and frozen at -84°C for one week. From each distal femur all tissues except cartilage and subcondral bone were removed and small pieces of normal osteochondral tissue (NOCT) were taken. The remaining osteochondral tissue has been demineralized in 0,6M HCl (Chem-Lab, Belgium) over night and again small pieces of demineralized osteochondral tissue (OCDT) were cutted with a scalpel and placed in a PBS solution for 24 hours. The remaining OCDT were separated in 4 groups. Two groups were decellularized in 0,5% and 1% SDS (Sigma, UK) and another two in 0,5% and 1% Triton X-100 (HiMedia, India). The decellularization lasted for 24 hours. At the next day the decellularized and demineralized osteochondral tissues (OCDDT) were washed with distilled water and PBS for 24 hours. All tissues were dessicated through centrifugation at 4000 rpm for 10 min (Hettich, Germany). From all types of OCT were cutted from three to nine pieces 20 mg each and quantification of DNA was performed with GeneJET Genomic DNA Purification Kit (Thermo Fisher, Lithuania). The results were read with spectrophotometer NanoDrop 2000c at wavelength of 260 nm (Thermo Fisher, USA). The best decellularized tissue and OCDT were tested for cytotoxicity with MTT test (ISO 10993-5) with mesenchymal stem cells and chondrocytes. Results. The average of DNA content in a rabbit NOCT is 36 ng/μl, in OCDT 4,23 ng/μl, OCDDT with 0,5% and 1% SDS is 3,23 ng/μl and 2,16 ng/μl respectively and in OCDDT with 0,5% and 1% TritonX-100 is 1,96 ng/μl and 0,96 ng/μl. At the MTT assay with mesenchymal stem cells and chondrocytes on the OCDT and OCDDT with 1% TritonX-100, we obtained a higher cell viability in both cases more than 80%. Conclusions. Obtaining a suitable osteochondral tissue for cartilaginous tissue engineering is very difficult because this process involves utilisation of a very toxic chemicals that harm this tissue. A shorter exposure period to chemical agents and preliminary modeling of the graft is mandatory. Also the OCDDT with 1% TritonX-100 shows the best results compared to others. en_US
dc.language.iso en en_US
dc.publisher MedEspera en_US
dc.subject graft en_US
dc.subject osteochondral en_US
dc.subject demineralized en_US
dc.subject decellularized en_US
dc.title Obtaining of a suitable osteochondral graft for articular cartilage engineering en_US
dc.type Article en_US


Files in this item

This item appears in the following Collection(s)

  • MedEspera 2018
    The 7th International Medical Congress for Students and Young Doctors, May 3-5, 2018

Show simple item record

Search DSpace


Advanced Search

Browse

My Account

Statistics