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The three-dimensional liver matrix for tissue engineering

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dc.contributor.author Jian, Mariana
dc.contributor.author Cobzac, Vitalie
dc.contributor.author Moghildea, Ion
dc.contributor.author Macagonova, Olga
dc.date.accessioned 2020-07-06T07:50:44Z
dc.date.available 2020-07-06T07:50:44Z
dc.date.issued 2018
dc.identifier.citation JIAN, Mariana, COBZAC, Vitalie, MOGHILDEA, Ion, MACAGONOVA, Olga. The three-dimensional liver matrix for tissue engineering. In: MedEspera: the 7th Internat. Medical Congress for Students and Young Doctors: abstract book. Chișinău: S. n., 2018, p. 201-202. en_US
dc.identifier.uri https://medespera.asr.md/wp-content/uploads/Abastract-Book-2018.pdf
dc.identifier.uri http://repository.usmf.md/handle/20.500.12710/10981
dc.description Tissue Engineering and Cell Cultures laboratory, Nicolae Testemitanu State University of Medicine and Pharmacy of the Republic of Moldova en_US
dc.description.abstract Introduction. According to The World Health Organization in 2012, about one-third of the world's population has serological evidence of hepatitis B infection (VHB). Terminal stage liver disease or hepatocellular carcinoma caused by VHB, leads to 0.5-1 million deaths per year. Worldwide viral hepatitis B is considered the 9th cause of death and represents 5-10% of all liver transplantation. That's why the phenomenon is perceived as significant global issues in public health. The growing of people number who need the liver transplant and the insufficiency of organ donors, as the advancement in bioengineering has enabled the development of new therapeutic strategies which involve generation of functional artificial organ, obtained by the decellularization technology and create extracellular matrix and their subsequent recellularisation. Aim of the study. To obtain a liver matrix by decellularization and to maintain its vascular tree. Materials and methods. As the object of this study served rat livers (n=9) which were subjected to decellularization with sodium dodecyl sulfate solution (SDS) 0.1 and 0.5% and the combination of sodium dodecyl sulfate 0.1% to 0.5% and anticoagulant. Subsequently, the extraction of nucleic acids was performed according to the protocol QIAamp Blood Mini Kit (2003). Results. After the liver tissue decellularization we obtained the liver matrix. The quantification of nucleic acids revealed the existence of a small amount of DNA 1.04 ± 0.43 ng/μl, * p<0,05 in decellularised matrix with SDS solution and anticoagulant. In case of decellularization by SDS exclusively, we obtained a larger amount of nucleic acids which revealed a less efficient decellularization 5.2 ± 2.19 ng/μl, * p <0.05. Conclusions. The use of detergent SDS with anticoagulant for decelularisation is more effective method in comparision with only SDS solution, which was proved by quantification of nucleic acids content in decellularised matrix. A more efficient decellularized liver tissue represent a 3D bioconstruction for future recellularisation. en_US
dc.language.iso en en_US
dc.publisher MedEspera en_US
dc.subject decellularization en_US
dc.subject recellularisation en_US
dc.subject liver matrix en_US
dc.title The three-dimensional liver matrix for tissue engineering en_US
dc.type Article en_US


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  • MedEspera 2018
    The 7th International Medical Congress for Students and Young Doctors, May 3-5, 2018

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