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dc.contributor.author Lazăr, Cornelia
dc.date.accessioned 2020-07-06T12:02:05Z
dc.date.available 2020-07-06T12:02:05Z
dc.date.issued 2018
dc.identifier.citation LAZĂR, Cornelia. Lipid peroxidation in experimental ovarian torsion. In: MedEspera: the 7th Internat. Medical Congress for Students and Young Doctors: abstract book. Chișinău: S. n., 2018, p. 199-200. en_US
dc.identifier.uri https://medespera.asr.md/wp-content/uploads/Abastract-Book-2018.pdf
dc.identifier.uri http://repository.usmf.md/handle/20.500.12710/11004
dc.description Department of Biochemistry and Сlinical Biochemistry, Nicolae Testemitanu State University of Medicine and Pharmacy of the Republic of Moldova en_US
dc.description.abstract Introduction. Ovarian torsion is a disease where ischemia/reperfusion injuries have a significant role. The detorsion is required to preserve the affected ovary. As it is known, if one organ is exposed to ischemia, the reperfusion may increase cells lesions due to the enhance of oxidative stress. One marker of lipids exposure to reactive oxigen species is malondialdehyde (MDA). Aim of the study. To determine the changes in MDA levels in ovarian homogenates of female rats that underwent various ovarian torsion/detorsion models and to appreciate the effects of simple and controlled detorsion (reperfusion) on the MDA levels. The experimental protocol was authorized by The Ethics Committee of the "Nicolae Testemitanu" State University of Medicine and Pharmacy. Materials and methods. The subjects of our research were 70 females rats (Rattus albicans). The animals were divided into seven groups (n=10): Group 1: no intervention; Group 2: the rats experienced laparotomy only; Group 3: the animals underwent ovarian torsion (ischemia) for 3 hours; Group 4: the rats have borne ovarian torsion for 3 hours succeeded by simple reperfusion for 1 hour; Group 5: the rats underwent 3 hours ovarian torsion and 1 hour controlled detorsion (assured by opening and closing the clips on the ovarian annexes in 10 seconds intervals for 120 seconds, succeeded by 1 hour of simple reperfusion); Group 6: the rats experienced 3 hours ovarian ischemia and 24 hours simple reperfusion; Group 7: the rats were exposed to 3 hours ovarian torsion and 24 hours controlled reperfusion. MDA levels were determined by Галактионова Л. П., et al. method (1998), modified by Gudumac V., et al. (2012). The results were analyzed using ANOVA. Results. The obtained MDA levels were statistically significant high in ischemia group, compared to those rats that underwent only laparatomy. The simple reperfusion groups had a statistically significant high levels of MDA compared to 24 hours controlled reperfusion group. Conclusions. Ovarian torsion and its detorsion involve reactive oxigen species production, that determines lipid peroxidation. Controlled detorsion can diminish this process and decrease the level of MDA that is produced. en_US
dc.language.iso en en_US
dc.publisher MedEspera en_US
dc.subject ovarian en_US
dc.subject torsion en_US
dc.subject malondialdehyde en_US
dc.title Lipid peroxidation in experimental ovarian torsion en_US
dc.type Article en_US


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  • MedEspera 2018
    The 7th International Medical Congress for Students and Young Doctors, May 3-5, 2018

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