dc.contributor.author |
Lazăr, Cornelia |
|
dc.date.accessioned |
2020-07-06T12:02:05Z |
|
dc.date.available |
2020-07-06T12:02:05Z |
|
dc.date.issued |
2018 |
|
dc.identifier.citation |
LAZĂR, Cornelia. Lipid peroxidation in experimental ovarian torsion. In: MedEspera: the 7th Internat. Medical Congress for Students and Young Doctors: abstract book. Chișinău: S. n., 2018, p. 199-200. |
en_US |
dc.identifier.uri |
https://medespera.asr.md/wp-content/uploads/Abastract-Book-2018.pdf |
|
dc.identifier.uri |
http://repository.usmf.md/handle/20.500.12710/11004 |
|
dc.description |
Department of Biochemistry and
Сlinical Biochemistry,
Nicolae Testemitanu State University of Medicine and Pharmacy of the Republic of Moldova |
en_US |
dc.description.abstract |
Introduction. Ovarian torsion is a disease where ischemia/reperfusion injuries have a significant
role. The detorsion is required to preserve the affected ovary. As it is known, if one organ is
exposed to ischemia, the reperfusion may increase cells lesions due to the enhance of oxidative
stress. One marker of lipids exposure to reactive oxigen species is malondialdehyde (MDA).
Aim of the study. To determine the changes in MDA levels in ovarian homogenates of female
rats that underwent various ovarian torsion/detorsion models and to appreciate the effects of
simple and controlled detorsion (reperfusion) on the MDA levels. The experimental protocol was
authorized by The Ethics Committee of the "Nicolae Testemitanu" State University of Medicine
and Pharmacy.
Materials and methods. The subjects of our research were 70 females rats (Rattus albicans).
The animals were divided into seven groups (n=10): Group 1: no intervention; Group 2: the rats
experienced laparotomy only; Group 3: the animals underwent ovarian torsion (ischemia) for 3
hours; Group 4: the rats have borne ovarian torsion for 3 hours succeeded by simple reperfusion
for 1 hour; Group 5: the rats underwent 3 hours ovarian torsion and 1 hour controlled detorsion
(assured by opening and closing the clips on the ovarian annexes in 10 seconds intervals for 120
seconds, succeeded by 1 hour of simple reperfusion); Group 6: the rats experienced 3 hours
ovarian ischemia and 24 hours simple reperfusion; Group 7: the rats were exposed to 3 hours
ovarian torsion and 24 hours controlled reperfusion. MDA levels were determined by
Галактионова Л. П., et al. method (1998), modified by Gudumac V., et al. (2012). The results
were analyzed using ANOVA.
Results. The obtained MDA levels were statistically significant high in ischemia group,
compared to those rats that underwent only laparatomy. The simple reperfusion groups had a
statistically significant high levels of MDA compared to 24 hours controlled reperfusion group.
Conclusions. Ovarian torsion and its detorsion involve reactive oxigen species production, that
determines lipid peroxidation. Controlled detorsion can diminish this process and decrease the
level of MDA that is produced. |
en_US |
dc.language.iso |
en |
en_US |
dc.publisher |
MedEspera |
en_US |
dc.subject |
ovarian |
en_US |
dc.subject |
torsion |
en_US |
dc.subject |
malondialdehyde |
en_US |
dc.title |
Lipid peroxidation in experimental ovarian torsion |
en_US |
dc.type |
Article |
en_US |