Significance of genexpert MTB/RIF method in the diagnostics of pulmonary tuberculosis

Abstract

Introduction:In the published literature, it is awarded a great deal of importance to the subject of TB diagnostic with the GeneXpert/RIF method, this being a fully closed automated system for M.Tuberculosis and resistant to RIF through the REAL TIME PCR technique. Purpose:The determination of the efficiency of the GeneXpert/RIF genetic-molecular method in the diagnosis of tuberculosis in comparison with the bacteriologic, microscopic methods.Resources and methods: Overall in Chisinau in 2014 were investigated 413 new cases of pulmonary tuberculosis. In the research were introduced 361 patients examined with the GeneXpert/RIF method. The positive result of the test showed 174 cases (48.2%). There were determined 123 cases (70.7%) GeneXpert/RIF positive-sensitive and 51 cases (29.3%) GeneXpert/RIF positive-resistant. Results:In comparison with the microscopic and bacteriologic methods, the sensitivity of the GeneXpert/RIF method was of 48.2%, specificity of 100%, 70.7% GeneXpert/RIF sensitive and 29.3% GeneXpert/RIF resistant. Conclusion: Priority of Xpert MTB/RIF molecular- methode has been confirmed by high sensitivity to the sputum microscopy, speed determination of resistance to RIF to bacteriological method, and the possibility of early treatment initiation in patients with MDR TB.