UV spectrophotometric method validation of analysis of a new substance with pronounced antimycobacterial activity

Abstract

Introduction. Tuberculosis is a curable disease and Antimycobacterial remedies are applied successfully more than 40 years. However, TB is a major public health problem, especially for developing countries, including Moldova. Increased incidence of tuberculosis requires the development and standardization of new concepts and therapeutic remedies.

Aim of the study. UV spectrophotometric method validation of a new antimycobacterial substance for quantitative analysis of its in pure substance.

Material and methods. Spectrophotometer UV (Agilent 8453); electronic balance (OHAUS), 1E-4,4-dimethyl-1-(4-nitrophenyl) -2- (1H-1,2,4, triazole-1-yl) -1-penten-3on.

Results. According to the study conducted, the absorption spectrum was analyzed using a spectrophotometer UV, highlighting absorption maxima at 201 nm and 301 nm. There were analyzed five different concentration ethanol solution and built calibration chart of the solution. They were determined and validated the following parameters: repeatability, specificity, accuracy, reproducibility. A defined mode and conditions necessary for conducting spectrophotometric analysis.

Conclusions. In the study were investigated the numerical values of some qualitative parameters of a new chemical molecule, which are the basis for further stability studies and formulation of medicinal forms.