Thin layer chromatographic separation of isohydrafural and methyluracyl in mechanical combination and ear drops

Abstract

INTRODUCTION. The combined ear drops represent an advantage over the monocomponent ones due to their polyvalent action. The thin layer chromatography (TLC) method is used to separate the active principles. The purpose. Separation and identification of isohydrafural and methyluracil in mechanical combination and pharmaceutical form by TLC. MATERIAL AND METHODS. Experimental series of isohydrafural (IHF), methyluracil (MU) (Sigma Aldrich, USA), chromatographic plates "Silufol", chromatography chamber, solvents, reagents according to the European Pharmacopoeia. RESULTS. Mobile phases were used: chloroform-acetone (70:30), 1-butanol-diethyl ether-acetone (10:85:5), ethyl acetatehexane (2:1), glacial acetic acid-water-butanol (1:1) 1:4), chloroform-methanol-glacial acetic acid (90:8:8 and 95:10:2). During the analyzes, 4 working techniques were used with the detection of spots in UV light at different wavelengths, spraying with pyridine and pdimethylaminobenzaldehyde solutions. The mobile phase ethyl acetate-hexane (2:1) was selected for the qualitative analysis of the compounds in mechanical mixture (I) and the pharmaceutical form (II). The values of the retention factors are: I-IHF-Rf = 0,37; MU-Rf = 0,72 and II-IHF-Rf = 0,35; MU-Rf = 0,70. CONCLUSIONS. The elaborated working technique will be included in the quality specification of the combined ear drops for the identification of IHF and MU.