dc.description.abstract |
INTRODUCTION. The combined ear drops represent an advantage
over the monocomponent ones due to their
polyvalent action. The thin layer chromatography
(TLC) method is used to separate the active
principles.
The purpose. Separation and identification of
isohydrafural and methyluracil in mechanical
combination and pharmaceutical form by TLC. MATERIAL AND METHODS. Experimental series of isohydrafural (IHF),
methyluracil (MU) (Sigma Aldrich, USA),
chromatographic plates "Silufol", chromatography
chamber, solvents, reagents according to the
European Pharmacopoeia.
RESULTS. Mobile phases were used: chloroform-acetone (70:30),
1-butanol-diethyl ether-acetone (10:85:5), ethyl acetatehexane (2:1), glacial acetic acid-water-butanol (1:1) 1:4),
chloroform-methanol-glacial acetic acid (90:8:8 and
95:10:2). During the analyzes, 4 working techniques were
used with the detection of spots in UV light at different
wavelengths, spraying with pyridine and pdimethylaminobenzaldehyde solutions. The mobile phase
ethyl acetate-hexane (2:1) was selected for the qualitative
analysis of the compounds in mechanical mixture (I) and
the pharmaceutical form (II). The values of the retention
factors are: I-IHF-Rf = 0,37; MU-Rf = 0,72 and II-IHF-Rf =
0,35; MU-Rf = 0,70. CONCLUSIONS. The elaborated working technique will be included in the quality specification of the combined ear drops for
the identification of IHF and MU. |
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