Abstract:
Introduction.
Thin layer chromatography (TLC) allows the separation of substances
from the mixture and has been used for the analysis of the
combination of ciprofloxacin (antimicrobial from the fluoroquinolone
group) and econazole (antifungal, imidazole derivative).
Purpose.
Separation and identification of ciprofloxacin and econazole in
combination by TLC, selection of suitable solvents for the detection of
both substances in combination.
Material and methods.
Pharmaceutical substances ciprofloxacin hydrochloride and econazole
nitrate (Sigma Aldrich, USA), chromatographic plates "Silufol",
chromatography chamber, solvents, reagents according to the
European Pharmacopoeia. Results.
Of the 28 chromatographic systems with different polarity
investigated, the solvent mixture Methanol: Acetonitrile (40:60) is the
optimal mobile phase for both substances, as it forms well-defined
spots and achieves a good separation of components. After
chromatography, the plates were sprayed with Dragendorf reagent and
examined in UV light at a wavelength of 254 nm (fluorescence and
yellow-orange spots). For ciprofloxacin hydrochloride Rf = 0.32, and
for econazole nitrate Rf=0,77.
Conclusions.
The working technique developed for the separation of ciprofloxacin and
econazole in combination by TLC can be used to identify substances in the
same pharmaceutical form.
Description:
Department of Pharmaceutical and Toxicological Chemistry ”Nicolae Testemitanu” SUMP, Chisinau, Republic of Moldova, Congresul consacrat aniversării a 75-a de la fondarea Universității de Stat de Medicină și Farmacie „Nicolae Testemițanu” din Republica Moldova, Ziua internațională a științei pentru pace și dezvoltare
