The thin layer chromatography application for identification of ciprofloxacine and econazole in combination

Abstract

Introduction. Thin layer chromatography (TLC) allows the separation of substances from the mixture and has been used for the analysis of the combination of ciprofloxacin (antimicrobial from the fluoroquinolone group) and econazole (antifungal, imidazole derivative). Purpose. Separation and identification of ciprofloxacin and econazole in combination by TLC, selection of suitable solvents for the detection of both substances in combination. Material and methods. Pharmaceutical substances ciprofloxacin hydrochloride and econazole nitrate (Sigma Aldrich, USA), chromatographic plates "Silufol", chromatography chamber, solvents, reagents according to the European Pharmacopoeia. Results. Of the 28 chromatographic systems with different polarity investigated, the solvent mixture Methanol: Acetonitrile (40:60) is the optimal mobile phase for both substances, as it forms well-defined spots and achieves a good separation of components. After chromatography, the plates were sprayed with Dragendorf reagent and examined in UV light at a wavelength of 254 nm (fluorescence and yellow-orange spots). For ciprofloxacin hydrochloride Rf = 0.32, and for econazole nitrate Rf=0,77. Conclusions. The working technique developed for the separation of ciprofloxacin and econazole in combination by TLC can be used to identify substances in the same pharmaceutical form.