Oxidative stress in evolution of endometriosis

Abstract

Spreading of endometriosis is succeeded by the destructive processes in the myocytes membrane, in the myometrium connective tissue, and also by the activation of lipid peroxidation processes. Objectives: the purpose of this work was to estimate the oxidative stress degree in patients with adenomyosis and to determine the oxidant/antioxidant potential of different treatment methods. Materials and methods: There were investigated 130 patients with adenomyosis in different stages of the disease, which were administered different methods of treatment. The oxidative stress degree was estimated by determining lipid peroxidation (POL) final product (malonic dialdehyde (DAM)), the serum antioxidant activity (AAO) and the enzymes: superoxide dismutase (SOD), catalase, and glutathione peroxidase (GP), and then calculated the antioxidant activity index AAO/DAM. Results: DAM dynamics are correlated with the severity of the disease, and in the 4th stage, it exceeds almost twice (p<0, 01) the physiological normative in the Republic of Moldova (4, 56±0, 09 nmol/l). The antioxidant activity index marks a similar tendency and this activity is suppressed as the disease progresses, in the 4th stage reaching 54, 83±0, 80% compared with the tolerable normative 74,28±0,48% (p<0,01). Conclusions: The antioxidant protection index AAO/DAM, which has been calculated in this work, can serve as a supplementary preclinical criterion to objectivize the adenomyosis severity, to predict the disease evolution and treatment efficacy. Hysterectomy - a radical method of treatment, changes the AAO/DAM index, but at the same time, it remains 2 times below the tolerable normative, persisting the risk of progression of the pathologic process, of spreading or malignization.