Introduction. The microbial biofilm-forming ability is one of the major aspects of the emerging issue of antibiotic resistance, which makes them tolerant to antibiotics and host defense
systems and other external stresses, thus contributing to persistent chronic infections. A series
of relevant studies confirmed the high efficiency of aminopropanol derivatives as potential
antibacterial and antifungal agents. This present study was aimed to evaluate the antimicrobial activity of new 1-[(2,4-(di-tert-butylphenoxy))-3-dialkylamino-2-propanol] derivatives
on the planktonic bacterial/fungal cells and biofilms.
Material and methods. The minimum inhibitory concentrations (MIC) of the new compounds
were determined by a standard method, along with their effects on biofilms estimated via the
gentian violet adsorption-desorption assay.
Results. The КVM-219 compound showed the most pronounced effect on planktonic bacterial
and fungal cells. The MIC values ranged between 0.78 μg/mL to 12.5 μg/mL, depending on the
microbial strain. The KVM-316 compound exhibited the strongest inhibitory effect on biofilms,
thus preventing their formation by S. aureus (96.1%), E. coli (57.2%), and P. aeruginosa
(96.1%).
Conclusions. The 15 newly synthesized 1-[(2,4-(di-tert-butylphenoxy))-3-dialkylamino-2-
propanol] derivatives revealed marked antibacterial and antifungal effects on planktonic microorganisms. Most of these compounds showed a strain-specific inhibition of biofilm formation by at least 50% for S. aureus 222, E. coli 311, P. aeruginosa 449 and C. glabrata 404
strains.
1-[(2,4(di-terț-butylphenoxy))-3-dialkylamino-2-propanol] asupra celulelor planctonice
bacteriene/fungice și asupra biofilmelor.
Material și metode. Concentrațiile minime inhibitorii (CMI) ale compușilor noi au fost determinate printr-o metodă standard, activitatea antibiofilm a fost testată prin absorbția violetului de gențiană pe structuri formate pe plăci de polistiren, urmată de resolubilizare cu solvent
organic și resazurină ca indicator redox.
Rezultate. Efectul cel mai pronunțat asupra celulelor planctonice bacteriene și fungice l-a
demonstrat compusul КVM - 219, CMI 0,67 μg/ml - 12,5 μg/ml, în funcție de microorganism,
iar asupra biofilmelor - compusul KVM-316. KVM-316 a prevenit formarea biofilmelor de
către S. aureus (96,1%), E. coli (57,2%) și P. aeruginosa (96,1%).
Concluzii. Cei 15 derivați nou sintetizați ai 1-[(2,4-(di-tert-butylphenoxy))-3-dialkylamino-2-propanol] au prezentat efecte antibacteriene și antifungice pronunțate asupra microorganismelor planctonice. Majoritatea acestor compuși au inhibat în mod specific formarea biofilmelor de către tulpinile clinice S. aureus 222, E. coli 311, P. aeruginosa 449 și C. glabrata
404 cu cel puțin 50%.
