Evaluation of protease-inhibitory blood system with gastroesophageal reflux disease in patients with diabetes type 2

Abstract

The aim of our study was to investigate the condition of proteinase-inhibitor system of blood with gastroesophageal reflux disease (GERD) in patients with diabetes mellitus (DM) type 2. Materials and methods: 7 patients with erosive GERD, combined with diabetes type 2 (1A group), 9 patients with nonerosive GERD with the diabetes type 2 (group IB) in the background, 7 patients with erosive GERD, with no endocrine diseases detected during a detailed examination (group 2A), 8 patients with nonerosive GERD (2B group). The control group consisted of 10 healthy individuals (PCC) due to the age (3rd group). The evaluation of proteolytic activity was carried out by determining the level of lysis of asoalbumin, asokazein and azokol. Results. It was established that in the absence of diabetes type 2 in patients with GERD occurs amplification lysis of small dispersed proteins to 94% in group 2A (p <0,05) and to 8.9 % in group 2B (p >0,05); lysis of large dispersed proteins - to 64 % (p <0,05) and to 19,4% (p >0,05); lysis of collagen - to 46 % (p <0,05) and to 16,2% (p > 0,05), according to USO. GERD with the diabetes type 2 (group IB) in the background (group IB) is accompanied by increased lysis of asoalbumin to 114% in group 1A (p <0,05) and to 13.8 % in group IB (p >0,05), lysis of asokazein to 109 % (p <0,05) and to 37,9% (p <0,05); azokol lysis to 47 % (p <0,05) and to 21,6% (p <0,05) according to the third group of patients. In patients with erosive GERD combined with diabetes type 2 and in the absence of it, the concentration of a2- macroglobulin ( MG ) significantly decreased to 36,1% (p <0,05) and to 29,6% (p <0,05) according to the USO. In groups IB and 2B, on the contrary, the level o f a2- MG increased compared with USO 2.5 times (p <0,05) and 2 times (p <0,05), with the probable difference between them (p <0,05). Conclusion. GERD is accompanied by increased proteolytic activity of plasma in the background of reduction of a2-M G (in the presence of erosions) and increased a2-MG content (in the form nonerosive GERD).