Abstract:
Introduction: Gemotherapy is based on using the so-called "stem cells", which are
embryonic plant tissues, namely young parts that hold the regenerating power of the plant. Plant
products used as sources of active ingredients with valuable therapeutic qualities are buds,
branches, internal bark or root bark of young branches, sap, seeds germinated and young seedlings.
Bioactive complex of these plant products can quickly intervene in human metabolism, producing
series of reactions at the molecular and cellular role of eliminating foreign substances that help
detoxify the locked cells (based on the specific action of certain organs) and resume their
functionality. Use of gemmoderivates shows a gentle therapy, with a deep action and without
negative consequences. Classical gemmotherapy was initiated by Belgian doctor Pol Henry (in
1950 ), which has withstood time and in the last decades show a rising interest.
Purpose: Qualitative and quantitative analysis of flavonosids and tannins in buds gemmae of
woody plants.
Materials and Methods: As a biological material for chemical study served buds of horse
chestnut trees, walnut, white birch, white poplar and cherry. Flavonosids qualitative study was
conducted by applying color or precipitation reactions and quantitative- spectrophotometer. Qualitative
analysis was performed by tannins specific reaction on TLC, here quantified by titrimetric method.
Results: The result of the chemical study of flavonosids in plant products gemmae of Aesculus
hippocastanum, Juglans regia, albosinensis Betula, Populus alba, Prunus avium established the presence
of the following constituents flavonoids. In the result of qualitative reactions we determined the
presence of flavones, flavonols, flavanonls, anthocyanins, aurons and chalcones in Pv gemmae. The
spectrophotometric study of flavonoids shows that the maximum content belong white poplar buds 7.208 %, then decreasing at Walnut - 6.808 % , cherry-3,508 % horse chestnut-2.912 % ,white birch2.746 % , birch-2.220 %. The specific reactions for tannins identification demonstrate the presence of
condensed tannins and hydrolyzable tannins in buds. The titrimetric dosage denotes the presence of a
high content in analyzed buds, most being in the horse chestnut buds ( 20.541 % ), followed by the
walnut ( 15.406 % ). The cherry buds contain ( 9.928 % ), followed by white poplar buds ( 6.847 %) and
white birch ( 6.505 % ) and the lowest content of tannins have the buts of white birch (2, 326 %).
Conclusions: Thus, the analyzed buts besides the specific biocomplex conditioned by the
presence of specific meristematic tissue may also contain large quantities of tannins and
flavonosides with valuable therapeutic qualities. The buds represents a complex biological entity ,
in which is realized a synergy of the components in the human body
Description:
Department of Pharmacognosy
and Pharmaceutical Botany, State University of Medicine and Pharmacy “Nicolae Testemitanu”,
Republic of Moldova