Abstract:
Introduction:
Rhodiola rosea L. (golden root) is a valuable medicinal
plant, which due to its precious properties is intensively
collected and is in danger of extinction in many regions of
the globe. The active components, characteristic of the
species R. rosea, accumulate mainly in rhizomes, having
adaptogenic, biostimulatory, and antioxidant properties. The
curative properties of this species are largely due to
phenylpropanoids (glycosides of cinnamic alcohol - rosavin,
rosarin, and rosin), phenylethanoloids (p-tyrosol and its
glycoside salidroside), and terpenes.
Purpose: The aim of the research was to determine the content
of rosavin and salidroside in ethanolic extracts from the
rhizomes of R. rosea plants in the Carpathian population,
Romania.
Material and methods: Ethanolic extracts (40-70% EtOH) were analyzed by
thin-layer chromatography (CSS) and UV-VIS spectrometry
at λmax 254 and 276 nm wavelengths, recalculated to rosavin
and salidroside.
Results: The intensity of the Rf bands characteristic of
rosavin and salidroside in the chromatogram of the
extract in the 40% ethyl alcohol solution was the
higherst. compared to that characteristic of other
extracts. Increasing the
concentration of ethyl
alcohol from 40% to 50, 60
and 70% led to a gradual
decrease in the intensity of
all components.
The results obtained by UV-VIS spectrometry reveal
that the rosavin content in R. rosea rhizome extracts is
1.25 ± 0.19%and salidroside 1.53 ± 0.98%.
Conclusions: Based on the data obtained by CSS and
spectrophotometric method, we can conclude that rosavin
and salidroside from R. rosea rhizomes are best extracted
in 40% ethyl alcohol solution, and the content of active
principles in rhizomes collected in the Carpathian
Mountains, Romania, falls within the characteristic data
for R. rosea rhizomes collected in other regions of the
Earth.
Description:
Institute of Genetics, Physiology and Plant Protection, Chisinau, Republic of Moldova
