Methods of dosing flavonoids in medical plants

Abstract

Introduction: Flavonoids represent a group of useful compounds of vegetal origin that are of great interest for physiotherapy and pharmacology. This is a class of phenolic substances which gives its color to many species of flowers and fruits. Frequently, these pigments can be found in such plants as glycosides, in which one or more of the hydroxy groups of phenols are combined with reducing glucose. Based on the analysis, the effects of flavonoids can be grouped around the following biochemical processes: antioxidant and anti-inflammatory effect, and an important influence for the function of the immune system against asthma and allergies; in general could change the inhibition functioning of enzymes, viruses and bacteria effect. These benefits argue the objective of the study: dosing flavonoids in different part of the plant throught spectrophotometric and chromatographic techniques. Purpose and objectives: To assess the methods of dosing flavonoids from various vegetable products: underground part, aerial parts, flowers, leaves and seeds. Materials and methods: Vegetable products containing flavonosids: Silybi fructus, Calendulae flores, Menthae piperitae herba; Agrimoniae herba, Simphyti radices. Reference Standards - quercetin, rutoside, luteolin, silibinin, hyperoside. The analyzes were performed at perchin Elmer spectrophotometer Lambda 25 UV-VIS and high pressure liquid chromatography Jasco reversed phase. Results: Flavonosids extraction was performed with ethyl alcohol 70%. For spectrophotometric determinations, were obtained extracts from the different part of the plant and analyzed in relation to alcoholic solutions of reference standards: quercetin, rutoside, luteolin, silibinin, hyperoside. The results were recalculated after standard dosage of quvercetin at wavelength 375 nm. Following results were obtained for the total content of flavonoids: Simphyti radices-0,08%; Agrimoniae herba-1,39%; Calendulae flores-0,26% Silybi fructus-0,09%; Menthae piperitae herba-0,95 %. For chromatographic separation was developed a unique technique for all extracts, based on the use of the solvent system acetonitrile:purified water (80:20). The final results of dosing flavonoids by HPLC method are correlated with those obtained from UV-VIS spectrophotometric determination. Conclusion: The obtained optimized extracts present the total concentrations of flavonoids, as evidenced by HPLC analysis and UV-VIS.