Influence of platelets rich plasma (PRP) on the regeneration of skin conditions allergic dermatitis

Abstract

Introduction: Modern approach to the problem of skin diseases characterized by the influence on the regeneration of tissues at the cellular level, leading to restoration of structure and function of the body as a whole. Aim: to examine the effects of PRP on regenerative properties of skin conditions allergic dermatitis. Materials and methods: Experiments were performed on female mice ICR, aged 3-4 months. PRP received by treating peripheral blood Machinery Smart Prep (Harvester Corp.). Animals were divided into two groups. The first group in plot area for 10 days rubbed potassium dichromate, then locally, intradermal injected into the alfected area PRP dose of 0.1 ml twice at intervals of 7 days. The second group of animals rubbed dichromate of potassium, during the same periodand then watched the self-healing skin. Animal deduced from the experiment on the 17th, 31st and 45th day. Conducted pathologic study involved skin using different methods of coloring material. Results: Macroscopically, visible damages to skin were not observed at the animals of the first group. The skin was covered with hair. In the morphological study of the skin revealed the preservation of its layer structure, satisfactory vascularization of the zone lesions, expressed basal layer, the structure of hair is not changed. Macroscopically, the skin of the animals from the second group was thin, with varying degrees of proliferation of connective tissue. Microscopically we detected acanthosis, small spongiosis, without bubbles, leukocyte infiltration of various degrees of severity, extension of epidermal outgrowths, areas of parakeratosis and phenomenon of acantholysis. Blood vessels had a small caliber. Conclusions: PRP promotes high quality and rapid reparative regeneration of skin, which helps preserve the morphological properties of tissue. The positive effect correction caused by several factors (cytokines and other biologically active substances), which enhance chemotaxis and proliferation of cellular elements in the lesion focus, and participate in the processes of adaptation.