Ischemic postconditioning and activity complement component C3 in cerebral ischemic and reperfusion injury

Abstract

Introduction: Ischemic postconditioning (IPost) is an effective mechanism to protect the cells from ischemic and reperfusion injury. Activation of the complement system in ischemic and reperfusion brain injury can cause additional damage to healthy tissue. Changes in the activity of C3 complement component in global cerebral ischemia and IPost are unknown. Purpose: The aim of this study was to quantify the functional activity of C3 component of complement in the serum of rats at different stages of reperfusion after cerebral global ischemia and IPost. Adult male rats Wistar weigting 250-280g were used for this study. Animals were housed in a 12hours/12hours light/dark cycle with free access to water and food. Transient global cerebral ischemia-reperfusion in the rat by reversible occlusion of major vessels, extended from the aortic arch and supplied the brain with blood. With the serum of rats determined The activity of complement component C3 on the second and seventh day of reperfusion period after a ten-minute global cerebral ischemia was determined in serum of rats. It was shown that reversible 10-minute ischemic brain injury in rats leads to increased activity of C3 component of complement in the first seven days after global ischemia, with the maximal increase in the C3 activity on the 2nd day of reperfusion. IPost leads to a significant increase in the functional activity of complement component C3 on the 7th day of reperfusion. This work was supported by the grant of the President of the Russian Federation for support of leading scientific groups 2359.2012.7.