Study of embryotoxic, fetotoxic and teratogenic properties of entomologic drugs

Abstract

Introduction: Embryotoxic and teratogenic properties of entomologic drugs were studied preclinically. Studied drugs are obtained from insects Lepidoptera, at different stages of metamorphoses (Imupurin- obtained from pupae, Entoheptin-from eggs, and Imuheptin-from eggs and pupae of Lepidoptera). Purpose and objectives: The research was conducted in two stages, with the aim: determining of embryo- development disorders (I step-antenatal, and II step-postnatal observations). Material and methods: Initially, we tested the embryotoxic and fetotoxic activities of tested drugs in rats. Tested substances were administered in 2 ml of 0,9% NaCl solution via a gastric tube, daily, at the same time; the control group received 2 ml of 0.9%NaCl solution. Daily observations not found behavioral deviations during pregnancy in females, included in the experimental groups in comparison with the control group. In the second step we evaluated the teratogenic action of tested substances, and postnatal development in the first 60 days of descendant’s life. Results: After administration of tested drugs, rats became slightly more active for 10 minutes, with subsequent recovery. Examination of skin, mucous membranes and hairiness showed no pathological changes. Body weight in rats of all groups increased an average with 30g. On the 20th day of pregnancy studied females were euthanized. It was studied preimplantation mortality index, which determines the difference between the number of corpora lutea in the ovaries and the number of implanted sites in the uterus, which is equal to 0. Then we calculated preimplantation index equal with the ratio of preimplantation places and the number of embryos, being equal to 0. Number of descendants born by primiparous females is on average 3-4 individuals, corresponding to the number of descendants born by females from vivarium. In all neonates were determined craniocaudal length and body weight, which are on average 3.74 g and 27.5 mm respectively. External examination revealed no abnormalities. Behavior of studied rats did not differ from the behavior in control group. Tooth eruption took place at the 8th day of life, hair growth on the 11-day, opening eyes to the 15-day, all corresponding age rats. At euthanasia of newborns developmental abnormalities were not detected. Conclusions: No embryotoxic effects were detected in tested drugs use. No differences in reproductive performance (conception and pregnancy) between experimental group and the control group. Thus, no embryotoxic, fetotoxic and teratogenic effects were seen in pregnant females treated with entomologie drugs.