Applying of physico-chemical methods in chemico-toxicological analysis of diclophenac

Abstract

The chimico-toxicological investigations on medicamentai preparations represent an essential problem. Their efficiency depends on isolation, dosage and identification of compounds by various phisico-chemical methods. Diclophenac is part of nonsteroidal antiinflamatory (NSAI) group used as an antiinflamatory, analgesic and antifebrile remedy. The exact mechanicm of action is not absolutely known, but it is believed that the primary mechanism responsible for the antiinflamatory, antifebrile and analgesic action is the inhibition synthesis of prostaglandin by inhibiting of cyclooxygenasis (COX)and itis likely to inhibit the synthesis of ADN. The inhibition of (COX) also decreases the prostaglandines from the gastric epithelium, making it more sensible to gastric acid corrosion.In this context there is a specific interest of studyingof diclophenac in biological fluids. As a consequence,we suggested to clear up those factors which influence the isolation of the compound from the blood plasma.We used chloroform as an extragent, which has a specific character for the nonionized forms from byological fluids. The pH value is important which gives the possibility to isolate the compound from biological fluid,its passing from ionized form in a molecular one, which encourages the efficacy of extraction with lipophilic solvents. Diclophenac was ectracted from blood plasma after acidulation with sulphuric acid(pH 2,5-3,0)and precipitation of proteins with treechloracetic acid, acid extracts under went spectrofotometric investigations at a wave lenght of 275 nm. We applied sillicotic plaques on thin layers within chromatography of the foil wing solvent system: Ethylacetate: methanol: amoniacal sol.25% (8:10:10), ethylacetate:chloroform:acetone4:l), methano:amonia 25%(100:1,5). We used the Draghendorff reagent and iodine fumes. These methods serve as a certain and efficient potential in chemico-toxicological analysis practice.