Abstract:
Pyroxicam (4 hydroxy-2 methyl-N-2 pyrydinil-2H-l,2 benzotiazin-3 carboxamide-1,1
dioxide) is used in medicine to treat the rheumatoid polyarthritis, anchylopoetic spondilitis, gout
attack, periarthritis, traumatic pains. It concerns to the group of drugs with antiinflamatory features,
analgesic. The action mechanism can be explained by the synthesis inhibition of prostaglandines and
the inhibition of the synthesis of cyclooxigenase and the migration of leucocytes to the inflammatory
focus inhibiting the phagocytosis and releasing of lysosomal hydrolysis. In certain conditions
(overdosage, hepatic diseases) pyroxicam possesses toxic features. In this context the study of
pyroxicam is a specific interst in biological fluids. As a result we intended to study the factors (the pH values of the medium the extragents polarizing acting during the process of isolation of the compound
from the blood serum). Pyroxicam has alkaline properties; it can be extracted from an alkali medium.
We used chloroform as an extraagent, which carries a specific character for the compounds in
unionized form from biological fluids. The pH value is important, which gives us the opportunity to
isolate pyroxicam from biological fluids, its passage from ionized form in a molecular one, which
encourages the officiency of extraction with lipophilic solvents. The pyroxicam was isolated from
blood serum after acidulation with oxalic acid (pH2.0-2.5) and the proteins sedimentation with
threechloracetic acid, then extracted from alkali medium with chloroform. Alkalic extracts underwent
spectrophotometric studies at a wave length 247 nm. We applied silicosis plaques on a thin layer
within chromatography in the following system of solvents: Chloroform: Acetone (4:1) Rf=0.51;
Ethylacetate: Methanol: Ammoniacal solution 25% (85:10:5) Rf=0.17; Ethylacetate Rf=0.38. These
methods can be applied in chemical toxicological analysis practice.Acetone (4:1) Rf=0.51;
Ethylacetate: Methanol: Ammoniacal solution 25% (85:10:5) Rf=0.17; Ethylacetate Rf=0.38. These
methods can be applied in chemical toxicological analysis practice.
