Abstract:
The goal of project was the elaboration of an alternative Azithromycin assay given the fact
that the Pharmacopoeial method is the biological one. This method is hardly realizable, and requires
special determination conditions, which also creates some difficulties in the substance and
pharmaceutical forms analysis. Materials and methods: For spectral analysis Agilent - 8453 UV-VIS
spectrophotometers in wavelengths range from 350 up to 550 nm were used. Phosphate buffer
solutions with different pH values were used as solvent. The colored product, resulting from reaction
with concentrated sulphuric acid, was investigated. Results: Azithromycin, which is a compound with
complex structure and is part of macrolide antibiotics group, forms a colored compound in reaction
with concentrated sulphuric acid. In order to determine the possibility of using this compound in the
UV-VIS spectrophotometric assay, we have studied the properties of mixture complex being
composed from Azithromycin and Sulphuric acid; such as parameters also were assayed: the stability
over the derivatization time, its dependency on reagents co-ratio and solvent’s influence, and the
value of pH environment. There were been developed the optimal technique of life time colouring
reaction: the amount of reagents - 10 ml; reaction time - 30 min; pH - 7.0. The spectrum of
absorption was recorded in the range from 350 up to 550 nm; the maximum of absorption was
established at 483 nm. The basic parameters of the method were determined: solution concentration,
specific absorption, absorbance law enforcement. The linearity and accuracy of the method were
evaluated that range within the limits of about 3%. Conclusions: 1. There were been established the
optimum conditions of Azithromycin assay, using the UV-VIS spectrophotomety on the base of
coloring reaction of Azithromycin with concentrated Sulphuric acid. 2. There were been developed
valuable technique and have evaluated the main criteria of validation: linearity, accuracy and the
repeatability of the method. 3. UV-VIS spectrophotometry of quantitative Azithromycin
determination may be also employed in the determination of this active principle in the
pharmaceutical forms.
