Abstract:
The objective was to create a new pharmaceutical form for external use - the combined
ointment with Izohidrafural and Methyluracil, which combines the antibacterial action of
Izohidrafural and the regenerating action of Methyluracil and also to elaborate the method of
extraction of active principles from the ointment. Materials and methods: Izohidrafuralum,
Methyluracilum, the excipients: Vaselinum album, Alcoholum cetylstearylicum, Propylenglycolum,
Glycerinum, Tween 80, PEG 400, Natrii laurylsulfas; the extragents: Dimethylformamidum, Natrii
hydroxidum 0.1mol/l and the mixture of these two substances; spectrophotometer Agilent 8453 UVVIS. Results: The combined ointment with Izohidrafural and Methyluracil can be used in treating skin
diseases, in surgery, obstetrics and gynecology, ophthalmology, proctology due to high efficiency and
good way of application. It was studied the concentrations of 0.1% for Izohidrafural and 5% for
Methyluracil which ensure a good availability and the maximum pharmacological effect. To create the optimal formulation of ointment, the active substances have been incorporated into various
excipients, of different nature. So it was investigated four compositions containing emulsion-type
ointment bases OAV and W/O. It was established the manufacturing technology flow of the ointment.
To select the optimal composition that would allow an effective therapeutic action and minimal side
effects, first of all it was elaborated the optimal extraction method and after that it was used the
dosage of active substances from the extract by UV-VIS spectrometry methods. So, it was analyzed
the extraction with solution of 0.1% sodium hydroxide, dimethylformamide and the mixture: solution
of 0.1% sodium hydroxide and dimethylformamide (1:3). It was recorded the absorption spectra in
the region 250nm-450nm in the case of extraction with solution of 0.1% sodium hydroxide, 250
400nm in the case of extraction with dimethylformamide and 250-450nm in case of extraction with
the mixture. Conclusions: 1 .Izohidrafural and Methyluracil have been incorporated into different
excipients. As a result, it was investigated four compositions containing emulsion-type ointment
bases O/W and W/O; 2.1t was selected the optimal manufacturing technology for the compositions;
3.1t was elaborated the extraction with solution of 0.1% sodium hydroxide, dimethylformamide and
the mixture of these two substances; 4.1t was concluded that the optimal extraction method is with
dimethylformamide.
