The assessment of gonadotoxic activity of Thiamethoxam pesticide in form of three generics

Abstract

The purpose of the study was to assess the influence of Thiamethoxam TG (technical grade) pesticide (insecticide-neonicotinoid) produced by three different manufactures (test substances T1, T2 and T3 each of them contains 95.5%, 95.4% and 97% of active ingredient respectively) on fertility parameters of Wistar Han male rats. The studies were performed exposing 40 Wistar Han male rats for each test substance in two different doses (low dose of 1.5 mg kg and high dose of 15 mg kg). The detection of functional state of the gonads and evaluation of the reproductive ability for males were performed in the end of exposure period. The reproductive parameters for intact females mated with exposed males were assessed on the 20th day of pregnancy. The test substances T1 and T3 in the maximum dose of 15 mg kg reveal signs of reproductive toxicity only for exposed males. The test substance T2 in the maximum dose in its turn reveal signs of reproductive toxicity for exposed males and intact females. Based on the results we can conclude that obtained effects are dose-dependent and no effect levels are the same for all test substances (1.5 mg kg). The observed additional embryotoxic effect at the maximum dose for the test substance T2 presumably is the result of the origin of the test substance.