Abstract:
Determination of potential genotoxic effects within generic pesticide studies is an obligatory requirement for justifying
their safety usage and evaluation of potential risks. In our
research center, we successfully conduct the recommended
standard mutagenicity test battery in comlience with GLP, which includes gene mutation tests in bacteria in vitro (fluctuation
Ames assay OECD 471) and gene mutation tests in mammalian
cells in vivo and in vitro (micronucleus assay OECD 475, 487 and
metaphase chromosomal aberration assay OECD 474). The aim
m
of the study was to research the potential mutagenic
effects of generic pesticides glyphosate 95.2% (Gly1),
glyphosate 95.6% (Gly2), glyphosate 95.0% (Gly3)
in screening fluctuation Ames assay using Sal
nella typhimurium strains TA98 and TA100 with and
without metabolic activation, preincubation in the
suspension was 90 min. Selection of concentrations
were based on preliminary experiment in pre-screening assay which was performed before the main
test. In the absence of cytotoxicity and precipitation
in preliminary experiment the following concentrations (5; 1; 0.2; 0.04; 0.008; 0.0016 mg/ml ) were
defined. As a result: obtained experimental data of
positive and negative controls were ranged with
own historical control. Validated XL template was
used to calculate the results. Our results showed
statistically significant absence of the mutagenic
effect of generic pesticides glyphosates Gly1, Gly2,
Gly3 in fluctuation Ames assay.
Description:
Laboratory of Experimental Toxicology and Mutagenesis,
L.I. Medved’s Research Center of Preventive Toxicology,
Food and Chemical Safety MH, Kiev, Ukraine, Prima Conferință științifico-practică națională cu participare internațională ”Siguranţa chimică și toxicologia la confluenţa dintre domenii” 24-25 noiembrie 2016 Chișinău, Republica Moldova
