Abstract:
Introduction. The inflammatory response, manifested as acute necrosis, is induced by ischemia
in infracted myocardium. Myocardial remodelling is one of the complications, which leads to
arrhythmias and heart failure. Interleukine-6 (IL-6) is a cytokine involved in tissue remodelling,
as well as in the pro- and anti-inflammatory response pathways. Post infarct it promotes myocyte
hypertrophy and myocardial dysfunction. In addition, IL-6 inhibits cardiomyocyte apoptosis.
Aim of the study. To evaluate serum and homogenate IL-6 level in isoproterenol-induced acute
myocardial infarction.
Materials and methods. Forty adult male rats (Ratta albicans) were divided into five groups: L1
– intact (n=11); L2 – control animals which were administrated NaCl 0.9% (n=11); L3 (n=6), L4
(n=6) and L5 (n=6) included the animals with experimental myocardial infarction, reproduced by
injecting subcutaneously isoproterenol hydrochloride 100 mg/kg (one dose). Rats were
anesthetized, and sacrificed at 6h, 24h and 7 days respectively. For IL-6 assessment, we use
standard Rat IL-6 ELISA kit (Beijing 4A Biotech Co. Ltd). The results were analyzed by
Kruskal-Wallis nonparametric test using SPSS version 23. Discussion
Results. The investigated groups have not presented any statistically significant difference
neither in homogenate IL-6 content (p = 0.098), no in serum IL-6 level (p = 0,322). At the same
time, higher amounts of both homogenate and serum IL-6 were registered in experimental
groups compared to intact and control groups.
Conclusions. Inflammation plays a significant role in the pathogenesis of myocardial ischemic
injury. Infarcted myocardium increases the production of IL-6. Increased IL-6 levels for a
prolonged time can indicate associated inflammation and elevated risk of second myocardial
infarction. Serum IL-6 level following AMI can be used for the inflammatory process
monitoring. In order to prove it the research should be enlarged, and statistical correlations will
be performed.
