Experimental myocadial infarction and Interleukine-6 modifications

Abstract

Introduction. The inflammatory response, manifested as acute necrosis, is induced by ischemia in infracted myocardium. Myocardial remodelling is one of the complications, which leads to arrhythmias and heart failure. Interleukine-6 (IL-6) is a cytokine involved in tissue remodelling, as well as in the pro- and anti-inflammatory response pathways. Post infarct it promotes myocyte hypertrophy and myocardial dysfunction. In addition, IL-6 inhibits cardiomyocyte apoptosis. Aim of the study. To evaluate serum and homogenate IL-6 level in isoproterenol-induced acute myocardial infarction. Materials and methods. Forty adult male rats (Ratta albicans) were divided into five groups: L1 – intact (n=11); L2 – control animals which were administrated NaCl 0.9% (n=11); L3 (n=6), L4 (n=6) and L5 (n=6) included the animals with experimental myocardial infarction, reproduced by injecting subcutaneously isoproterenol hydrochloride 100 mg/kg (one dose). Rats were anesthetized, and sacrificed at 6h, 24h and 7 days respectively. For IL-6 assessment, we use standard Rat IL-6 ELISA kit (Beijing 4A Biotech Co. Ltd). The results were analyzed by Kruskal-Wallis nonparametric test using SPSS version 23. Discussion Results. The investigated groups have not presented any statistically significant difference neither in homogenate IL-6 content (p = 0.098), no in serum IL-6 level (p = 0,322). At the same time, higher amounts of both homogenate and serum IL-6 were registered in experimental groups compared to intact and control groups. Conclusions. Inflammation plays a significant role in the pathogenesis of myocardial ischemic injury. Infarcted myocardium increases the production of IL-6. Increased IL-6 levels for a prolonged time can indicate associated inflammation and elevated risk of second myocardial infarction. Serum IL-6 level following AMI can be used for the inflammatory process monitoring. In order to prove it the research should be enlarged, and statistical correlations will be performed.