Determination of metronidazole and miconazole nitrate in a mixture by spectrophotometric method

Abstract

Introduction. Metronidazole and miconazole nitrate are substances with antiprotozoal, antifungal and antibacterial contents. For quality control of these drugs, in particular, quantitative determination, the European Pharmacopoeia recommends the HPLC method. Aim of the study. Development of an express method for the quantitative determination of a mixture of metronidazole and miconazal nitrate based on the spectrophotometric method. Materials and methods. In the work was used an Agilent 8453 single-beam spectrophotometer from Hewlett Packard, USA. As standard (control) substances, the pharmaceutical substances metronidazole and miconazole were used, the content of the main substance in which was not lower than 98%. Results. The dependence of the absorption intensity in the ultraviolet (200-400 nm) and visible (400-760 nm) spectral regions in various solvents was studied. Conclusions. The obtained spectra demonstrated the possibility of analysis of the studied substances in the joint presence.