The total content of polyphenols in dry extracts from different parts of Hypericum perforatum L.

Abstract

Introduction. Hypericum perforatum L. belonging to the family Hypericaceae is a reputed medicinal plant including a wide range of important phytochemical components. The major components are: chlorogenic acid, rutin, hyperoside, quercitrin, quercetin, pseudohypericin, hypericin and hyperforin. Crude extract and individual compounds of H. perforatum have been reported to exert antidepressant, antibiotic, and antitumor activities. Getting of dry extracts is beneficial in terms of rational use of plant products, because the extraction yield of biologically active compounds is maximum, which also determines their high therapeutic properties. Aim of the study. Quantitative determination of total polyphenols and flavonoids in dry extracts from aerial parts, flowers and seeds of H. perforatum L. Materials and methods. The aerial parts, flowers and seeds of H. perforatum L. have been collected from the spontaneous flora and shade-dried. The dry extracts have been obtained through fractional maceration method. It was used as solvent ethanol 80%. The concetration of the extracts was done with the rotative evaporator Laborota 4011. Quantitative analysis of the phenolic compounds was realized using the Metertech UV/VIS SP 8001 Spectrophotometer. Results. The total of flavonoids and polyphenols in the dry extracts from flowers (57,10 and 105,04 mg/ml) is higher than in the aerial parts (38,24 and 42,63 mg/ml) and the seeds (14,04 and 32,39 mg/ml). The total polyphenol content was estimated using Folin-Ciocalteau reagent. The concentration of flavonoids and polyphenols was calculated from a standard curve plotted with known concentration of rutin and gallic acid. Conclusions. There is a need for further chemical study of plant materials Hyperici flores and Hyperici semina, therefore, these parts of the plant can be used as future vegetal products.