Abstract:
Introduction: Currently, the issue of the treatment of the teeth with endodontic periodontitis
is payed a particular attention. AThe main purpose of endodontics is the ensure permanent sterility
of the macro and micro dental root canals and the creation conditions for maintaining sterility in the
future. The intracanal use of solutions enriched with ozone currently presents a contemporary
method of treatment of chronic apical periodontitis. The process of endodontic space with ozonated
physiological solution will increase the chances of success during the treatment of chronic apical
periodontitis the introduction of ozonated serum (solution) in the periapical space will also increase
the chances that the tooth to be tolerated.
Purpose and Objectives: The inactivation with ozone of microflora involved in chronic
apical periodontitis.
Materials: Ozonator JQ type-589, thermostat TC-80 M-2, autoclave, spirtiera, bacteriological
loop, needle length 100 mm and the diameter cannula of 5 mm, boxes Pietri, bacteriological tubes,
bacteriological medium nutrient (agar, blood, sodium chloride), microbial culture - Streptococcus
(3- hemolyticus of clinical material. Methods: In the bacteriological tube was poured 4 ml of sodium chloride 0,9%, then with
bacteriological loop was suspensioned the Streptococcus (T- hemolyticus culture, until the turbidity
of 0,5 units according to Me Farland standard.The obtained suspension was divided into 2 tubes in
equal amounts. In the tube nr.2 was introduced a needle of length 100 mm and the diameter cannula
of 5 mm, connected through the rubber tube to ozonator. When the needle was introduced
simultaneously was set the time of exhibition 2 minutes. After this from both tubes, which was
subject to ozonary and which was not subject was done the seeding with bacteriological loop on
blood agar in the Petri box divided into 2 sections. The boxes with environments were placed in the
thermostat 18-24 hours, temperature 36±l°C.The recording results were visually performed
counting the number of colonies growing from both suspensions (with and without ozone). On the
sector without ozone no.l grew 350 colonies, on the sector with ozone no.2 grew 3 colonies.
Conclusion: Was demonstrated the antimicrobial activity of the ozone. The action time of
ozone used by us is sufficient to inactivate microorganisms. The application of physiological
ozonated solution in the treatment of chronic apical periodontitis will greatly enhance the
effectiveness of treatment.
