The use of allotropic form of oxygen in the treatment of chronic apical periodontitis

Abstract

Introduction: Currently, the issue of the treatment of the teeth with endodontic periodontitis is payed a particular attention. AThe main purpose of endodontics is the ensure permanent sterility of the macro and micro dental root canals and the creation conditions for maintaining sterility in the future. The intracanal use of solutions enriched with ozone currently presents a contemporary method of treatment of chronic apical periodontitis. The process of endodontic space with ozonated physiological solution will increase the chances of success during the treatment of chronic apical periodontitis the introduction of ozonated serum (solution) in the periapical space will also increase the chances that the tooth to be tolerated. Purpose and Objectives: The inactivation with ozone of microflora involved in chronic apical periodontitis. Materials: Ozonator JQ type-589, thermostat TC-80 M-2, autoclave, spirtiera, bacteriological loop, needle length 100 mm and the diameter cannula of 5 mm, boxes Pietri, bacteriological tubes, bacteriological medium nutrient (agar, blood, sodium chloride), microbial culture - Streptococcus (3- hemolyticus of clinical material. Methods: In the bacteriological tube was poured 4 ml of sodium chloride 0,9%, then with bacteriological loop was suspensioned the Streptococcus (T- hemolyticus culture, until the turbidity of 0,5 units according to Me Farland standard.The obtained suspension was divided into 2 tubes in equal amounts. In the tube nr.2 was introduced a needle of length 100 mm and the diameter cannula of 5 mm, connected through the rubber tube to ozonator. When the needle was introduced simultaneously was set the time of exhibition 2 minutes. After this from both tubes, which was subject to ozonary and which was not subject was done the seeding with bacteriological loop on blood agar in the Petri box divided into 2 sections. The boxes with environments were placed in the thermostat 18-24 hours, temperature 36±l°C.The recording results were visually performed counting the number of colonies growing from both suspensions (with and without ozone). On the sector without ozone no.l grew 350 colonies, on the sector with ozone no.2 grew 3 colonies. Conclusion: Was demonstrated the antimicrobial activity of the ozone. The action time of ozone used by us is sufficient to inactivate microorganisms. The application of physiological ozonated solution in the treatment of chronic apical periodontitis will greatly enhance the effectiveness of treatment.