Abstract:
Introduction.
Dioxoindolinone – (1'-(2-oxo-propil)-spiro[[1,3]dioxolane-2',3'-indolin]-
2'-one) – is a new native compound of the isatin group, synthesized in the
Organic Synthesis and Biopharmaceutical Laboratory of the Institute of
Chemistry.
The substance has anti-depressive and sedative activity and is researched
in order to develop methods of analysis and standartization.
Purpose.
Selection of chromatographic parameters for the elaboration of the
method of analysis of Dioxoindolinone (1 '- (2-oxo-propyl) -spiro
[[1,3] dioxolane-2', 3'-indoline] -2'-one), by HPLC method.
Material and methods.
Dioxoindolinone, experimental synthesis series; and possible specific
impurities (izatine – initial synthetic substance, ketal izatine –
intermediate product )were assessed; Shimadzu LC-20AD liquid
chromatograph with UV-detector SPD-20A, solvents and reagents in
accordance with the requirements of the European Pharmacopoeia.
Results.
The research allowed to select the optimal conditions for the analysis
of Dioxoindolinone by HPLC method: mobile phase – methanol: water:
phosphoric acid solution 0.1% (40:20:40), Zorbax Eclipse Plus C18
column, 5 μm, 4.6 x 250 mm; UV-VIS detector, wavelength 258 nm.,
column temperature 30 0C, beginning of the mobile phase 1.2 mL /
min, injection volume 20 μl. The retention times were: for
Dioxoindolinone – 6.5 min.; for izatine – 4,3 min.; for ketal izatine –
7,11 min.
Conclusions. The selected chromatographic conditions and mobile phase offer
possibilities for the elaboration of the method for the determination of
related substances in Dioxoindolinone, as well as its assay.
Description:
Universitatea de Stat de Medicină şi Farmacie „Nicolae Testemiţanu”, Catedra de chimie farmaceutică şi toxicologică, Chișinău, RM, Universitatea de Medicină și Farmacie „Carol Davila”, Facultatea de Farmacie, Disciplina Farmacologie și farmacie clinică, București, România
