Abstract:
Introduction: The high prevalence and the constant increase of incidence of skin fungal infections
and its annexes causes a continuing interest for the analysis and standardization of antifungal agents.
Purpose and objectives: Validation of UV spectrophotometric method for analysis of nitrate
Isoconazole obtained through original from synthetically imidazole ionic liquids.
Materials and methods: Reference study (articles of journals, periodicals, European
Pharmacopoeia). The object of the research was the sample of Isoconazole nitrate, synthesized in
the Laboratory of Synthetic Organic Chemistry Institute, Academy of Sciences of Moldova.
Instruments: UV Visible Spectrophotometer (Agilent 8453); electric balance (OHAUS).
Results: The Isoconazole nitrate solution spectrum shows the well-defined spectral
absorption maximum between 274 nm and 282 nm. Ethanol shows absorption maxima at other
wave lengths. So it can be used as a solvent. According to the calibration straight lines, a better
linearity shows the data taken at X 274 nm. In the concentration range 50-150 mg / mL, the relative
standard deviation is 0.22% maximum, which is equivalent to a very good accuracy of the method.
Conclusion: According to the results of the study, the UV spectrophotometric method was
validated. It allows the quantitative determination of the nitrate Isoconazole in ethanol solution. This
method is rapid, requiring small amounts of reagents, providing accurate and reproducible data.
Description:
Pharmaceutical and Toxicological Chemistry Department, State University of Medicine and
Pharmacy “Nicolae Testemi^anu”, Chisinau, Republic of Moldova
