Development and validation of the UV spectrophotometric method for analysis of ethanol solution of Isoconazol nitrat

Abstract

Introduction: The high prevalence and the constant increase of incidence of skin fungal infections and its annexes causes a continuing interest for the analysis and standardization of antifungal agents. Purpose and objectives: Validation of UV spectrophotometric method for analysis of nitrate Isoconazole obtained through original from synthetically imidazole ionic liquids. Materials and methods: Reference study (articles of journals, periodicals, European Pharmacopoeia). The object of the research was the sample of Isoconazole nitrate, synthesized in the Laboratory of Synthetic Organic Chemistry Institute, Academy of Sciences of Moldova. Instruments: UV Visible Spectrophotometer (Agilent 8453); electric balance (OHAUS). Results: The Isoconazole nitrate solution spectrum shows the well-defined spectral absorption maximum between 274 nm and 282 nm. Ethanol shows absorption maxima at other wave lengths. So it can be used as a solvent. According to the calibration straight lines, a better linearity shows the data taken at X 274 nm. In the concentration range 50-150 mg / mL, the relative standard deviation is 0.22% maximum, which is equivalent to a very good accuracy of the method. Conclusion: According to the results of the study, the UV spectrophotometric method was validated. It allows the quantitative determination of the nitrate Isoconazole in ethanol solution. This method is rapid, requiring small amounts of reagents, providing accurate and reproducible data.