Abstract:
Introduction. Uncoupling agents, or uncouplers, are molecules, which are classified as ion transporters,
located in the internal membrane of mitochondria. They discharge the proton gradient, which is generated
by the respiratory chain, by transporting hydrogen ions. As a result, ATP-synthetase cannot be activated
due to the lack of that gradient, thus the oxidative phosphorylation is "uncoupled" from the electron
transport chain. Therefore, the energy used for ATP synthesis is dissipated in heat, which explains the main
role of thermogenesis. There are 2 types of uncouplers: natural, which are UCPs (short for Uncoupling
Proteins) and synthetic, which have an importance in research and not only.
Aim of study. Exploring the roles and flaws of the uncoupling agents and the factors which increase their
activities
Methods and materials. Selected from articles, found in databases (PubMed, NCIB, Google Scholar, etc)
according to the keywords "uncoupling", "uncoupling agent", "oxidative phosphorylation".
Results. The most known and most studied uncoupling agent at the moment is thermogenin (UCP 1), which
is present mostly in the brown adipose tissue in newborns and hibernating mammals. It is also involved in
energetic balance due to its involvement in uncoupling oxidative phosphorylation from the respiratory
chain. The main stimulating factor of thermogenin is exposure to cold temperatures. Besides thermogenesis,
uncoupling agents have significant roles even in other organs. For example, in the nervous system, they
stimulate the transport of calcium ions outside mitochondria, thus avoiding neuronal hyperexcitability,
which is a key feature in epilepsy. Also, they help the neuronal tissue recover from injuries. Another
interesting effect scientists noticed about uncouplers is weight loss. Dinitrophenol, a synthetic uncoupling
agent, was used as a miracle pill for losing weight in the 1930s until FDA illegalized it in 1938 due to
severe side effects. The increase of an uncoupling protein can inhibit beta cells from endocrine pancreas to
synthesize insulin, leading to their dysfunction or even to type 2 diabetes. Another point about the
uncouplers is that the main factors that increase their activities are free fatty acids and thyroid hormones.
Conclusion. Uncoupling agents are not only molecules that generate heat, but have a major influence
towards some processes such as neuronal excitability, insulin synthesis, weight loss and so on. At the
moment, the scientists are still studying the recently discovered uncouplers, whose roles are still unknown.
