Abstract:
The experimental toxic hepatopathy (HP) was modeled by long term administration of
CCl4. It was established that CCl4 caused pronounced disorders of carbohydrate metabolism
manifested by the increased level of the metabolites – lactate, pyruvate and malate, and changes
in tissue enzyme activity – the increased activity of lactate dehydrogenase (LDH) and the
reduction of the functionality of the main dehydrogenases of the Krebs cycle – NADP-dependent
malate dehydrogenase (NADP-MDH), NADP-dependent isocitrate dehydrogenase (ICDHNADP)
and succinate dehydrogenase (SDH). The administration of local biologically active
compounds (BAC) – CMD-8, CMJ-23 and CMJ-33, of Spirulina Platensis sulphatated
polysaccharides (SSP) and of the remedy BioR Fe-Se in the toxic HP exerted important
influences both on the evolution of metabolites content changes and on the activity of the
investigated enzymes. The tested BAC succeeded to reduce the intensity of the toxic effects of
CCl4 on hepatocytes by inducing a tendency of normalization and even by restoring to normal
levels of the carbohydrate metabolites content, as well as of the functionality of studied cellular
enzymes.
Pe animale de laborator s-a modelat hepatopatia (HP) toxică prin administrarea de durată a
CCl4. S-a stabilit că CCl4 a provocat tulburări pronunţate ale metabolismulu glucidic care s-au
manifestat prin creşterea nivelului de metaboliţi – lactat, piruvat şi malat şi modificări ale
activităţii enzimelor tisulare – creşterea activităţii lactatdehidrogenazei (LDH) şi reducerea
funcţionalităţii principalelor dehidrogenaze ale ciclului Krebs – malatdehidrogenazei NADPdependente
(MDH-NADPd), izocitrat dehidrogenazei NADP-dependente (ICDH-NADPd) şi
succinatdehidrogenazei (SDH). Administrarea compuşilor biologic activi (CBA) autohtoni –
CMD-8, CMJ-23 şi CMJ-33, polizaharidelor sulfatate din spirulină şi a remediului BioR Fe-Se în
HP toxică a exercitat influenţe importante atât asupra modificărilor conţinutului de metaboliţi,
cât şi a dinamicului de activitate a enzimelor investigate. CBA luaţi în studiu au reuşit să reducă
intensitatea efectelor toxice ale CCl4 asupra hepatocitelor prin tendinţa de normalizare şi chiar
readucerea la valori normale a nivelului de metaboliţi glucidici, precum şi a funcţionalităţii
enzimelor celulare studiate.
