Abstract:
Introduction. Basil essential oil has been demonstrated to possess various beneficial properties,
including analgesic, anti-inflammatory, antibacterial, hepatoprotective, and immunomodulatory
effects. The multifunctionality of Basil oil makes it an important candidate for incorporation into
different dosage forms. The massive use of antibiotics has resulted in the emergence of resistant
microorganisms, which is another problem affecting public health. Pseudomonas aeruginosa,
Staphylococcus aureus, Salmonella spp, Staphylococcus aureus, Shigella spp., Enterococcus spp.,
Escherichia coli are the main bacteria that have developed this resistance. Plants and other natural
resources can provide a wide range of complex and structurally different compounds.
Aim of study. To determine the antibacterial activity of basil oil.
Methods and materials. The antimicrobial activity of Basil essential oil was assessed against
various indicator strains using the filter paper disc diffusion method. The in vitro testing of the
samples was performed on ATCC strains from the collection of the Microbiology Laboratory of
Saarland University on Escherichia coli (ATCC 25923) and S. epidermidis (ATCC 25922) strains.
The strains were revived by overnight growth in Brain-Heart Infusion broth (Oxoid, CM1135) at
37°C and were diluted to an optical density of 0.5 McFarland standard (1.5 x 108 CFU/ml).
Results. The data obtained from the disc diffusion method, followed by measurement of MIC,
indicate that E. coli and S. epidermidis showed lower values of MIC (1.25 mg/mL, respectively).
Therefore, the Basil oil was shown to exhibit strong antimicrobial activity against all
microorganisms tested, both Gram-positive and Gram-negative bacteria, even though Gram-positive strains seem to be more sensitive to it. Overall, the observed antimicrobial activity of Basil
oil might be attributed to the high contents of linalool that possesses a stronger antimicrobial
activity against Gram-positive bacteria than against Gram-negative bacteria.
Conclusion. Basil oil is a natural source that can be used in formulation of dosage forms with
antibacterial activity.
